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pcmv vsv g  (Addgene inc)


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    Structured Review

    Addgene inc pcmv vsv g
    Pcmv Vsv G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 3102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv+vsv+g/pCMV-VSV-G+(Plasmid+%238454)/pmc12907852-54-26-27
    Average 96 stars, based on 3102 article reviews
    pcmv vsv g - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Produced:

    Article Title: Identification of the mitochondria-related gene IRF7 shared by physical exercise and Parkinson’s disease and exploration of its potential molecular mechanisms
    Article Snippet: .. Lentiviral particles were produced using a third-generation packaging system with the constructed PLKO.1 or pCMV vectors, plus pCMV-VSV-G (8454; Addgene, Watertown, MA, USA), and psPAX2 (12260; Addgene, Watertown, MA, USA) plasmids. ..

    Article Title: LUCID-EV: a robust and quantitative bioluminescent assay for the detection of EV cytosolic delivery in the absence of VSV-G expression
    Article Snippet: .. Lentiviruses were produced by transfection of HEK293 FT cells with TransIT-293 according to manufacturer instructions with a mix of pCMV-VSV-G (Addgene 8454), pPAX2 (Addgene 12260), and lentiviral vector. ..

    Construct:

    Article Title: Identification of the mitochondria-related gene IRF7 shared by physical exercise and Parkinson’s disease and exploration of its potential molecular mechanisms
    Article Snippet: .. Lentiviral particles were produced using a third-generation packaging system with the constructed PLKO.1 or pCMV vectors, plus pCMV-VSV-G (8454; Addgene, Watertown, MA, USA), and psPAX2 (12260; Addgene, Watertown, MA, USA) plasmids. ..

    Cell Culture:

    Article Title: In vivo multiplexed modeling reveals diverse roles of the TBX2 subfamily and Egr1 in Kr as -driven lung adenocarcinoma
    Article Snippet: .. HEK293T cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum and transfected with each of our barcoded Lenti-sgRNA/Cre plasmids, along with pCMV-VSV-G (Addgene #8454) envelope plasmid and pCMV-dR8.2 dvpr (Addgene #8455) packaging plasmid, using polyethylenimine. ..

    Modification:

    Article Title: In vivo multiplexed modeling reveals diverse roles of the TBX2 subfamily and Egr1 in Kr as -driven lung adenocarcinoma
    Article Snippet: .. HEK293T cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum and transfected with each of our barcoded Lenti-sgRNA/Cre plasmids, along with pCMV-VSV-G (Addgene #8454) envelope plasmid and pCMV-dR8.2 dvpr (Addgene #8455) packaging plasmid, using polyethylenimine. ..

    Transfection:

    Article Title: In vivo multiplexed modeling reveals diverse roles of the TBX2 subfamily and Egr1 in Kr as -driven lung adenocarcinoma
    Article Snippet: .. HEK293T cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum and transfected with each of our barcoded Lenti-sgRNA/Cre plasmids, along with pCMV-VSV-G (Addgene #8454) envelope plasmid and pCMV-dR8.2 dvpr (Addgene #8455) packaging plasmid, using polyethylenimine. ..

    Article Title: LUCID-EV: a robust and quantitative bioluminescent assay for the detection of EV cytosolic delivery in the absence of VSV-G expression
    Article Snippet: .. Lentiviruses were produced by transfection of HEK293 FT cells with TransIT-293 according to manufacturer instructions with a mix of pCMV-VSV-G (Addgene 8454), pPAX2 (Addgene 12260), and lentiviral vector. ..

    Article Title: Enhancing Proteasome Activity in T Cells Alleviates Exhaustion and Improves Antitumor Immunity
    Article Snippet: .. Briefly, HEK 293T cells were transfected with lentiviral vectors and 187 packaging plasmids pRSV-REV (RRID:Addgene_12253), pMDLg/pRRE (RRID: 188 Addgene_12251), and pCMV-VSV-G (RRID: Addgene_8454) using jetPEI (PolyPlus 189 #101000053). .. Lentiviral supernatants were collected 48 hours later, filtered through a 0.45 μm 190 filter (VWR #514-0063), and concentrated with LentiX-Concentrator (Clontech #631231) 191 according to the manufacturer’s protocol.

    Article Title: Establishment of a green fluorescent protein (GFP)-based reporter for picornaviral 3C proteases.
    Article Snippet: The resulting plasmid, RUIPW, was subsequently digested with BamHI, and the SCARB2 sequence synthesized by Eurofins Japan was inserted. .. The resultant plasmids (RUIPW SCARB2), pMDLg/pRRE (Addgene plasmid # 12251), pRSV-Rev (Addgene plasmid #12253), and pCMV-VSV-G (Addgene plasmid #8454) were transfected into HEK293T cells using TransIT-LT1 Transfection Reagent (Mirus) according to the manufacturer’s protocol. ..

    Plasmid Preparation:

    Article Title: In vivo multiplexed modeling reveals diverse roles of the TBX2 subfamily and Egr1 in Kr as -driven lung adenocarcinoma
    Article Snippet: .. HEK293T cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum and transfected with each of our barcoded Lenti-sgRNA/Cre plasmids, along with pCMV-VSV-G (Addgene #8454) envelope plasmid and pCMV-dR8.2 dvpr (Addgene #8455) packaging plasmid, using polyethylenimine. ..

    Article Title: LUCID-EV: a robust and quantitative bioluminescent assay for the detection of EV cytosolic delivery in the absence of VSV-G expression
    Article Snippet: .. Lentiviruses were produced by transfection of HEK293 FT cells with TransIT-293 according to manufacturer instructions with a mix of pCMV-VSV-G (Addgene 8454), pPAX2 (Addgene 12260), and lentiviral vector. ..

    Article Title: Plasma extracellular vesicle modulate immune cell transcriptional responses following acute myocardial infarction
    Article Snippet: pMDLg/pRRE , Addgene , Plasmid #12251. .. pCMV-VSV-G , Addgene , Plasmid #8454. .. pLV[shRNA]-EGFP:T2A:Puro-U6>{hsa-miR-320b} , Vector Builder , Ecoli(VB250302-1277jxs).

    Article Title: Targeting PI3Kδ suppresses pancreatic cancer by dual disruption of fibrosis and immune evasion.
    Article Snippet: .. The pMSCVhygro-Cre (Addgene plasmid # 34565) was a gift from Kai Ge.61 The tet-pLKO-puro was a gift from Prof. Dmitri Wiederschain (Addgene plasmid #21915).60 The pLKO.1 puro (Addgene plasmid #8453), pCMV-VSV-G (Addgene plasmid #8454) and pCMV-dR8.2 dvpr (Addgene plasmid #8455) were a gift from Prof. Bob Weinberg.59 The shRNAs were obtained from Sigma Aldrich and cloned into the Tet-pLKO-puro, pLKO.1 puro or pLKO.1 Hygro plasmid after digestion with AgeI/EcoRI. ..

    Article Title: Establishment of a green fluorescent protein (GFP)-based reporter for picornaviral 3C proteases.
    Article Snippet: The resulting plasmid, RUIPW, was subsequently digested with BamHI, and the SCARB2 sequence synthesized by Eurofins Japan was inserted. .. The resultant plasmids (RUIPW SCARB2), pMDLg/pRRE (Addgene plasmid # 12251), pRSV-Rev (Addgene plasmid #12253), and pCMV-VSV-G (Addgene plasmid #8454) were transfected into HEK293T cells using TransIT-LT1 Transfection Reagent (Mirus) according to the manufacturer’s protocol. ..

    Clone Assay:

    Article Title: Targeting PI3Kδ suppresses pancreatic cancer by dual disruption of fibrosis and immune evasion.
    Article Snippet: .. The pMSCVhygro-Cre (Addgene plasmid # 34565) was a gift from Kai Ge.61 The tet-pLKO-puro was a gift from Prof. Dmitri Wiederschain (Addgene plasmid #21915).60 The pLKO.1 puro (Addgene plasmid #8453), pCMV-VSV-G (Addgene plasmid #8454) and pCMV-dR8.2 dvpr (Addgene plasmid #8455) were a gift from Prof. Bob Weinberg.59 The shRNAs were obtained from Sigma Aldrich and cloned into the Tet-pLKO-puro, pLKO.1 puro or pLKO.1 Hygro plasmid after digestion with AgeI/EcoRI. ..



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