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pcmv vsv g stewart  (Addgene inc)


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    Structured Review

    Addgene inc pcmv vsv g stewart
    Pcmv Vsv G Stewart, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 3117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv+vsv+g/pCMV-VSV-G+(Plasmid+%238454)/pm42789384-245-38-42
    Average 96 stars, based on 3117 article reviews
    pcmv vsv g stewart - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Produced:

    Article Title: Identification of the mitochondria-related gene IRF7 shared by physical exercise and Parkinson’s disease and exploration of its potential molecular mechanisms
    Article Snippet: .. Lentiviral particles were produced using a third-generation packaging system with the constructed PLKO.1 or pCMV vectors, plus pCMV-VSV-G (8454; Addgene, Watertown, MA, USA), and psPAX2 (12260; Addgene, Watertown, MA, USA) plasmids. ..

    Article Title: LUCID-EV: a robust and quantitative bioluminescent assay for the detection of EV cytosolic delivery in the absence of VSV-G expression
    Article Snippet: .. Lentiviruses were produced by transfection of HEK293 FT cells with TransIT-293 according to manufacturer instructions with a mix of pCMV-VSV-G (Addgene 8454), pPAX2 (Addgene 12260), and lentiviral vector. ..

    Construct:

    Article Title: Identification of the mitochondria-related gene IRF7 shared by physical exercise and Parkinson’s disease and exploration of its potential molecular mechanisms
    Article Snippet: .. Lentiviral particles were produced using a third-generation packaging system with the constructed PLKO.1 or pCMV vectors, plus pCMV-VSV-G (8454; Addgene, Watertown, MA, USA), and psPAX2 (12260; Addgene, Watertown, MA, USA) plasmids. ..

    Article Title: Targeting tryptophan hydroxylase 1 restricts growth and suppresses plasticity in neuroendocrine prostate cancer
    Article Snippet: .. Briefly, 293T cells 517 were co-transfected with a TPH1- or Dox-inducible TPH1 shRNA-expressing lentiviral construct, pCMV delta 518 R8.2 (Addgene), and pCMV-VSV-G (Addgene) in a 4:2:1 ratio using Lipofectamine 3000 reagent (Thermo 519 Fisher Scientific) following the manufacturer’s instructions. ..

    Cell Culture:

    Article Title: In vivo multiplexed modeling reveals diverse roles of the TBX2 subfamily and Egr1 in Kr as -driven lung adenocarcinoma
    Article Snippet: .. HEK293T cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum and transfected with each of our barcoded Lenti-sgRNA/Cre plasmids, along with pCMV-VSV-G (Addgene #8454) envelope plasmid and pCMV-dR8.2 dvpr (Addgene #8455) packaging plasmid, using polyethylenimine. ..

    Modification:

    Article Title: In vivo multiplexed modeling reveals diverse roles of the TBX2 subfamily and Egr1 in Kr as -driven lung adenocarcinoma
    Article Snippet: .. HEK293T cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum and transfected with each of our barcoded Lenti-sgRNA/Cre plasmids, along with pCMV-VSV-G (Addgene #8454) envelope plasmid and pCMV-dR8.2 dvpr (Addgene #8455) packaging plasmid, using polyethylenimine. ..

    Transfection:

    Article Title: In vivo multiplexed modeling reveals diverse roles of the TBX2 subfamily and Egr1 in Kr as -driven lung adenocarcinoma
    Article Snippet: .. HEK293T cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum and transfected with each of our barcoded Lenti-sgRNA/Cre plasmids, along with pCMV-VSV-G (Addgene #8454) envelope plasmid and pCMV-dR8.2 dvpr (Addgene #8455) packaging plasmid, using polyethylenimine. ..

    Article Title: LUCID-EV: a robust and quantitative bioluminescent assay for the detection of EV cytosolic delivery in the absence of VSV-G expression
    Article Snippet: .. Lentiviruses were produced by transfection of HEK293 FT cells with TransIT-293 according to manufacturer instructions with a mix of pCMV-VSV-G (Addgene 8454), pPAX2 (Addgene 12260), and lentiviral vector. ..

    Article Title: Loss of ABCA3 disrupts lipid balance and leads to AMPK-dependent suppression of SREBP1 in glioblastoma stem cells.
    Article Snippet: Malignant cancers exhibit distinct lipid metabolic features that support tumor initiation and progression.. Glioblastoma (GBM) is an aggressive brain tumor driven by GBM stem cells (GSCs), which are responsible for tumor development and therapy resistance.. However, effective treatments targeting vulnerable metabolic pathways in GSCs have not yet been developed.

    Article Title: HCMV US30 induces TMED10-DRP1-mediated mitochondrial fission and apoptosis to promote viral multiplication.
    Article Snippet: .. Then, HEK293 cells were transfected with the indicated retroviral plasmids, along with packaging plasmids psPAX2 (Addgene, 12260) and pCMV-VSV-G (Addgene, 8454). ..

    Plasmid Preparation:

    Article Title: In vivo multiplexed modeling reveals diverse roles of the TBX2 subfamily and Egr1 in Kr as -driven lung adenocarcinoma
    Article Snippet: .. HEK293T cells were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum and transfected with each of our barcoded Lenti-sgRNA/Cre plasmids, along with pCMV-VSV-G (Addgene #8454) envelope plasmid and pCMV-dR8.2 dvpr (Addgene #8455) packaging plasmid, using polyethylenimine. ..

    Article Title: LUCID-EV: a robust and quantitative bioluminescent assay for the detection of EV cytosolic delivery in the absence of VSV-G expression
    Article Snippet: .. Lentiviruses were produced by transfection of HEK293 FT cells with TransIT-293 according to manufacturer instructions with a mix of pCMV-VSV-G (Addgene 8454), pPAX2 (Addgene 12260), and lentiviral vector. ..

    Article Title: Loss of ABCA3 disrupts lipid balance and leads to AMPK-dependent suppression of SREBP1 in glioblastoma stem cells.
    Article Snippet: Malignant cancers exhibit distinct lipid metabolic features that support tumor initiation and progression.. Glioblastoma (GBM) is an aggressive brain tumor driven by GBM stem cells (GSCs), which are responsible for tumor development and therapy resistance.. However, effective treatments targeting vulnerable metabolic pathways in GSCs have not yet been developed.

    Article Title: Targeting PI3Kδ suppresses pancreatic cancer by dual disruption of fibrosis and immune evasion.
    Article Snippet: .. The pMSCVhygro-Cre (Addgene plasmid # 34565) was a gift from Kai Ge.61 The tet-pLKO-puro was a gift from Prof. Dmitri Wiederschain (Addgene plasmid #21915).60 The pLKO.1 puro (Addgene plasmid #8453), pCMV-VSV-G (Addgene plasmid #8454) and pCMV-dR8.2 dvpr (Addgene plasmid #8455) were a gift from Prof. Bob Weinberg.59 The shRNAs were obtained from Sigma Aldrich and cloned into the Tet-pLKO-puro, pLKO.1 puro or pLKO.1 Hygro plasmid after digestion with AgeI/EcoRI. ..

    Over Expression:

    Article Title: Loss of ABCA3 disrupts lipid balance and leads to AMPK-dependent suppression of SREBP1 in glioblastoma stem cells.
    Article Snippet: Malignant cancers exhibit distinct lipid metabolic features that support tumor initiation and progression.. Glioblastoma (GBM) is an aggressive brain tumor driven by GBM stem cells (GSCs), which are responsible for tumor development and therapy resistance.. However, effective treatments targeting vulnerable metabolic pathways in GSCs have not yet been developed.

    Knockdown:

    Article Title: Loss of ABCA3 disrupts lipid balance and leads to AMPK-dependent suppression of SREBP1 in glioblastoma stem cells.
    Article Snippet: Malignant cancers exhibit distinct lipid metabolic features that support tumor initiation and progression.. Glioblastoma (GBM) is an aggressive brain tumor driven by GBM stem cells (GSCs), which are responsible for tumor development and therapy resistance.. However, effective treatments targeting vulnerable metabolic pathways in GSCs have not yet been developed.

    In Vitro:

    Article Title: Loss of ABCA3 disrupts lipid balance and leads to AMPK-dependent suppression of SREBP1 in glioblastoma stem cells.
    Article Snippet: Malignant cancers exhibit distinct lipid metabolic features that support tumor initiation and progression.. Glioblastoma (GBM) is an aggressive brain tumor driven by GBM stem cells (GSCs), which are responsible for tumor development and therapy resistance.. However, effective treatments targeting vulnerable metabolic pathways in GSCs have not yet been developed.

    shRNA:

    Article Title: Targeting tryptophan hydroxylase 1 restricts growth and suppresses plasticity in neuroendocrine prostate cancer
    Article Snippet: .. Briefly, 293T cells 517 were co-transfected with a TPH1- or Dox-inducible TPH1 shRNA-expressing lentiviral construct, pCMV delta 518 R8.2 (Addgene), and pCMV-VSV-G (Addgene) in a 4:2:1 ratio using Lipofectamine 3000 reagent (Thermo 519 Fisher Scientific) following the manufacturer’s instructions. ..

    Clone Assay:

    Article Title: Targeting PI3Kδ suppresses pancreatic cancer by dual disruption of fibrosis and immune evasion.
    Article Snippet: .. The pMSCVhygro-Cre (Addgene plasmid # 34565) was a gift from Kai Ge.61 The tet-pLKO-puro was a gift from Prof. Dmitri Wiederschain (Addgene plasmid #21915).60 The pLKO.1 puro (Addgene plasmid #8453), pCMV-VSV-G (Addgene plasmid #8454) and pCMV-dR8.2 dvpr (Addgene plasmid #8455) were a gift from Prof. Bob Weinberg.59 The shRNAs were obtained from Sigma Aldrich and cloned into the Tet-pLKO-puro, pLKO.1 puro or pLKO.1 Hygro plasmid after digestion with AgeI/EcoRI. ..

    Retroviral:

    Article Title: HCMV US30 induces TMED10-DRP1-mediated mitochondrial fission and apoptosis to promote viral multiplication.
    Article Snippet: .. Then, HEK293 cells were transfected with the indicated retroviral plasmids, along with packaging plasmids psPAX2 (Addgene, 12260) and pCMV-VSV-G (Addgene, 8454). ..



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